abstract：:The mechanism by which hCG increases the rat testicular vascular permeability was studied by injecting the testes with hCG (0.1-100 ng) together with testicular interstitial fluid (150 mul) or with the fluid alone (control) and measuring the uptake of i.v. injected [125I]hCG and the interstitial fluid volume in the te...

journal_title：Molecular and cellular endocrinology

authors： Veijola M,Rajaniemi H

更新日期：1986-05-01 00:00:00

abstract：:The present study examined the influence of the calcium ionophores A23187 and X537A on the calcitonin (CT) secretory process. The isolated perfused porcine thyroid was used to evaluate ionophore effects on CT secretion and thyroid slices were used to measure 45Ca uptake. Both A23187 and X537A enhanced the rate of CT r...

journal_title：Molecular and cellular endocrinology

authors： Pento JT

更新日期：1986-04-01 00:00:00

abstract：:The high-density lipoprotein (HDL) pathway in rat adrenocortical cells was studied at the electron microscopic level in vitro via colloidal gold labelling. Steroid hormone assays were performed to confirm that the cells remained intact, viable, responsive to ACTH under the applied conditions, and to reveal the steroid...

journal_title：Molecular and cellular endocrinology

authors： Tóth IE,Szabó D,Bácsy E,Szalay KS,Hesz A,Szollár LG

更新日期：1986-02-01 00:00:00

abstract：:We have used a new methodology to generate a monospecific antiserum to the corticotropin (ACTH) receptor on mouse Y-1 adrenal cells. Using immunoaffinity chromatography the ACTH receptor was purified, and the molecular structure and 125I-ACTH binding characteristics were determined. A molecular weight (Mr) of 225 000 ...

journal_title：Molecular and cellular endocrinology

authors： Bost KL,Blalock JE

更新日期：1986-01-01 00:00:00

abstract：:Thyrotropin-releasing hormone (TRH) stimulates maximally both the release of previously synthesized prolactin and the de novo synthesis of prolactin by GH4C1 rat pituitary cells at concentrations less than those necessary to fully occupy the TRH receptor at equilibrium. We have examined the dependency of maximal TRH-e...

journal_title：Molecular and cellular endocrinology

authors： Ramsdell JS,Tashjian AH Jr

更新日期：1985-12-01 00:00:00

abstract：:Primary cultures of ovine thyroid cells were induced to differentiate by addition of thyrotropin (TSH). This was demonstrated as an accumulation of 2 thyroid-specific proteins, thyroglobulin and thyroid peroxidase, using immunofluorescent staining methods and immunoprecipitation of biosynthetically labeled cultures. A...

journal_title：Molecular and cellular endocrinology

authors： Errick JE,Eggo MC,Burrow GN

更新日期：1985-11-01 00:00:00

abstract：:As part of a study concerning the mechanism of mammotroph autoregulation, the intracellular fate of [125I]iodo rat PRL, internalized by mammotrophs, was determined by quantitative electron microscope autoradiography. Simple grain density (GD) analysis of mammotroph autoradiograms showed that 5 cellular compartments (p...

journal_title：Molecular and cellular endocrinology

authors： Giss BJ,Walker AM

更新日期：1985-10-01 00:00:00

abstract：:We investigated the effect of pretreatment of immature rats with 5 or 50 micrograms nafoxidine (UA), or with 0.05 micrograms 17 beta-estradiol (E2) on several uterine responses elicited by treatment with a test injection of 15 micrograms E2, administered 48 h after pretreatment. Early (6 h) and late (24 h) responses w...

journal_title：Molecular and cellular endocrinology

authors： Galand P,Tchernitchin N,Tchernitchin AN

更新日期：1985-10-01 00:00:00

abstract：:Basal and gonadotropin stimulated adenylate cyclase activity was assessed in testicular tissues obtained from men (20-80 years). A disparity was observed in the gonadotropin responsiveness of the human testicular adenylate cyclase system to hFSH and hCG stimulation. Of the tissues analyzed, 61% were FSH responsive and...

journal_title：Molecular and cellular endocrinology

authors： Berman MI,Anand-Srivastava MB,Sairam MR

更新日期：1985-08-01 00:00:00

abstract：:The stimulatory effects on Leydig cell testosterone secretion of a polypeptide(s) factor present in testicular interstitial fluid (IF) were compared with those of hCG and an LHRH agonist (LHRH-A). The actions of IF and LHRH-A were similar in showing (1) a delayed onset of action, (2) enhancement of testosterone produc...

journal_title：Molecular and cellular endocrinology

authors： Sharpe RM

更新日期：1985-07-01 00:00:00

abstract：:In light of current discussions on multiple forms of inhibin, it was thought of interest to ascertain the identity of the postulated 'iso-hormones' of bull seminal plasma inhibin (Chari et al., 1978). By subjecting the biologically active fraction, obtained by Sephadex G-100 gel filtration of bull seminal plasma aceto...

journal_title：Molecular and cellular endocrinology

authors： Mohapatra SK,Duraiswami S,Chari S

更新日期：1985-07-01 00:00:00

abstract：:In cultured human lymphocytes (IM-9) and in isolated rat adipocytes human growth hormone is substrate for a receptor-mediated degradation. When the cells are incubated with monoiodinated human growth hormone half of the radioactivity dissociating from the cells is in the form of [125I]monoiodotyrosine. Since IM-9 lymp...

journal_title：Molecular and cellular endocrinology

authors： Weyer B,Sonne O

更新日期：1985-06-01 00:00:00

abstract：:The effect of thyroid status on the postnatal development of the two molecular forms of Na+,K+-ATPase, distinguished kinetically on the basis of their ouabain sensitivity, was examined in rat brain. Hypothyroidism induced by PTU from day 1 postnatally significantly reduced the Na+,K+-ATPase activity in cerebellum (22-...

journal_title：Molecular and cellular endocrinology

authors： Atterwill CK,Reid J,Athayde CM

更新日期：1985-05-01 00:00:00

abstract：:The actions of ACTH to regulate the synthesis of the various enzymes involved in steroid hormone biosynthesis have been studied using bovine adrenocortical cells in monolayer culture. ACTH causes an increase in the synthesis of both the mitochondrial and the microsomal forms of cytochrome P-450 involved in steroid hor...

journal_title：Molecular and cellular endocrinology

authors： Waterman MR,Simpson ER

更新日期：1985-02-01 00:00:00

abstract：:We have used plus-minus hybridization to identify Xenopus liver cDNA clones of mRNAs whose levels are regulated by estrogen. One clone identified in this way was shown to be a nearly full-length cDNA clone of the mRNA coding for a small 22 000 dalton estrogen-inducible serum protein (EISP). Quantitation of EISP mRNA l...

journal_title：Molecular and cellular endocrinology

authors： Hayward MA,Barton MC,Shapiro DJ

更新日期：1985-02-01 00:00:00

abstract：:The fibromuscular stroma (FMS) separated from the seminal vesicles of adult guinea pigs incorporated [35S]methionine into a 12 500 X g soluble fraction in vitro. Incorporation decreased 60% in tissues from 7-day castrates with no further change up to 21 days post-castration. Administration of 5 alpha-dihydrotestostero...

journal_title：Molecular and cellular endocrinology

authors： Weinberger MJ

更新日期：1984-12-01 00:00:00

abstract：:Very few silver grains were seen on the luteal cell surface and none intracellularly after incubation for 2 h at 4 degrees C with 10 nM [3H]prostaglandin (PG) E1 or [3H]PGF2 alpha. However, incubation at 22 degrees C or 38 degrees C for 2 h resulted in association of numerous grains on the luteal cell surface as well ...

journal_title：Molecular and cellular endocrinology

authors： Chegini N,Rao CV,Cobbs G

更新日期：1984-12-01 00:00:00

abstract：:At 14 days of age, hypothyroid and normal rats are rendered hypoglycaemic by insulin injection and the subsequent induction in adrenal tyrosine hydroxylase (TH) is studied. Hypothyroidism impairs both the intensity and the time-course of adrenal TH induction. ...

journal_title：Molecular and cellular endocrinology

authors： Gripois D,Valens M

更新日期：1984-11-01 00:00:00

abstract：:Incorporation of p-chloromercuribenzene sulphonate (PCMBS) (1mM) in the assay medium for rat hepatic lactogenic receptor produced complete inhibition of binding of [125I]oPRL to the membrane. However, the presence of the thiol-reactive agent produced no effect on the binding of [125I]bGH to rat hepatic somatogenic rec...

journal_title：Molecular and cellular endocrinology

authors： Tsim KW,Cheng CH

更新日期：1984-11-01 00:00:00

abstract：:An enzyme system capable of monodeiodinating T4 to T3 and to rT3 in a microsomal fraction of normal human thyroid tissue is characterized. The pH optimum for 5'-monodeiodination of T4 to T3 was around 7.0 The Km (apparent) for the conversion of T4 to T3 was 5.7 mumoles/l. PTU, ipodate and rT3 inhibited T4 deiodination...

journal_title：Molecular and cellular endocrinology

authors： Boye N,Laurberg P

更新日期：1984-10-01 00:00:00

abstract：:This study demonstrates that a single subcutaneous injection of gonadotrophin-releasing hormone (GnRH) (60 ng) to GnRH-deficient (hpg) male mice causes a doubling of pituitary GnRH receptors (GnRH-R). No change in GnRH-R occurs during the time of LH release (15-60 min) or up until 4 h post-GnRH. Between 4 and 12 h the...

journal_title：Molecular and cellular endocrinology

authors： Detta A,Naik SI,Charlton HM,Young LS,Clayton RN

更新日期：1984-09-01 00:00:00

abstract：:The seminal vesicles of male rat secrete tissue-specific proteins under androgenic control. The effects of testosterone on the rough endoplasmic reticulum (RER) and Golgi-system of this tissue have been quantified using specific antibodies. Castration was followed within 2 weeks by a 10-fold reduction in RER-specific ...

journal_title：Molecular and cellular endocrinology

authors： Fawell SE,Higgins SJ

更新日期：1984-08-01 00:00:00

abstract：:Phospholipase C (PHL-C) activity determined in homogenates of fat cells treated with physiological concentrations of insulin showed a 2-3-fold increase as compared to controls in the absence of insulin. The changes of PHL-C and pyruvate dehydrogenase (PDH) activity which was measured concomitantly exhibited very simil...

journal_title：Molecular and cellular endocrinology

authors： Koepfer-Hobelsberger B,Wieland OH

更新日期：1984-06-01 00:00:00

abstract：:The effects of tamoxifen (Tam) and its 4-hydroxylated metabolite (OH-Tam) on the growth of two human breast cancer cell lines ( MCF7 and BT20 ) were evaluated by fluorometric DNA assay. The effects of the antiestrogens were dependent upon their concentrations and the nature of the cells. At concentrations below 4 micr...

journal_title：Molecular and cellular endocrinology

authors： Bardon S,Vignon F,Derocq D,Rochefort H

更新日期：1984-05-01 00:00:00

abstract：:The effects of cytochalasin D on basal and adrenocorticotropin (ACTH)-stimulated steroid production as well as stress fiber organization by bovine adrenocortical (BAC) cells grown in monolayer culture have been investigated. Corticosteroid and pregnenolone release was determined by use of radioimmunoassay. The additio...

journal_title：Molecular and cellular endocrinology

authors： Rainey WE,Shay JW,Mason JI

更新日期：1984-05-01 00:00:00

abstract：:The effects of a GnRH antagonist analogue (N-acetyl-Ala1,D-p-Cl-Phe2,D-Trp3,6-GnRH, Ant.) and a GnRH antiserum (A/S) on the development of pituitary-testicular function were studied in immature (23/24-31/32-day-old) rats. In another experiment the Ant. treatment was combined with bromocriptine (BR)-induced hypoprolact...

journal_title：Molecular and cellular endocrinology

authors： Huhtaniemi IT,Stewart JM,Channabasavaiah K,Fraser HM,Clayton RN

更新日期：1984-02-01 00:00:00

abstract：:Explants from the mammary gland of 6 lactating cows were cultured in M-199 medium containing insulin (1.0 micrograms/ml) and hydrocortisone (0.5 micrograms/ml) and supplemented with bPRL (0.2-1.0 micrograms/ml) or oGH (0.1, 0.5 and 2.5 micrograms/ml) or bGH or hGH (0.2 and 1.0 micrograms/ml). It was found that neither...

journal_title：Molecular and cellular endocrinology

authors： Gertler A,Cohen N,Maoz A

更新日期：1983-12-01 00:00:00

abstract：:Tissue slices or dispersed cells of bovine parathyroid gland were incubated with [3H]leucine to label the intracellular proteins and then tested for their secretory response to isoproterenol and cycloheximide at different calcium concentrations. Secretion of the newly synthesized as well as the older PTH and SP-I was ...

journal_title：Molecular and cellular endocrinology

authors： Chu LL,MacGregor RR,Hamilton JW

更新日期：1983-12-01 00:00:00

abstract：:The diterpene forskolin was found to activate the adenylate cyclase system in intact tissue and membrane preparations of the immature rat ovary. The cyclic AMP (cAMP) response reached a maximal level after 5 min and no decline was observed even after 4 h of incubation. Forskolin stimulated production of both progester...

journal_title：Molecular and cellular endocrinology

authors： Hedin L,Rosberg S

更新日期：1983-11-01 00:00:00

abstract：:The main objective of this study was to characterize nuclear T3 and T4 binding in the developing rat brain. More specifically, we sought to determine (a) whether T3 and T4 bind to the same nuclear receptor, (b) whether there are multiple forms of nuclear T3 or T4 receptors, and (c) whether the above parameters are sim...

journal_title：Molecular and cellular endocrinology

authors： Margarity M,Matsokis N,Valcana T

更新日期：1983-08-01 00:00:00

abstract：:This study was aimed at testing the hypothesis that epidermal growth factor (EGF) can modulate the gonadotropin-dependent differentiation of theca-interstitial cells in vitro. Addition of EGF to chemically defined culture medium severely antagonized the ability of hCG to transform undifferentiated theca-interstitial c...

journal_title：Molecular and cellular endocrinology

authors： Erickson GF,Case E

更新日期：1983-07-01 00:00:00

abstract：:Polyamines are well-known ubiquitous components of living cells. Although these polycations have been implicated in the regulation of major cellular functions such as DNA, RNA and protein synthesis occurring during cellular proliferation and/or differentiation processes, their mechanism of action at the molecular leve...

journal_title：Molecular and cellular endocrinology

authors： Cochet C,Chambaz EM

更新日期：1983-06-01 00:00:00

abstract：:Poly(A+)-containing mRNA from human term placenta was used to direct protein synthesis in a nuclease-treated rabbit reticulocyte lysate, which is dependent on mRNA and tRNA for maximal activity. The major protein product was human pre-placental lactogen (hPL). Addition of tRNA from rabbit liver, rabbit reticulocyte, h...

journal_title：Molecular and cellular endocrinology

authors： Kelly SJ,Loria J,Gyves MT,Ilan J

更新日期：1983-02-01 00:00:00

abstract：:Modifications of tRNAs in various physiological or experimental conditions are well documented. We have compared isoacceptor tRNAs extracted from target organs (heart and liver) from thyroidectomized rats to those of control animals. Nine liver aminoacyl-tRNAs and eight heart aminoacyl-tRNAs from thyroidectomized and ...

journal_title：Molecular and cellular endocrinology

authors： Kessous C,Befort JJ,Befort N,Benmiloud M

更新日期：1983-02-01 00:00:00

abstract：:Forskolin, a diterpene hypotensive drug, activates adenylate cyclase in brain and in some other tissues (Seamon et al., 1981). Forskolin activated adenylate cyclase in particulate preparations and enhanced cyclic AMP accumulation in slices of dog thyroid. These effects were maximal within minutes and remained constant...

journal_title：Molecular and cellular endocrinology

authors： Van Sande J,Cochaux P,Mockel J,Dumont JE

更新日期：1983-01-01 00:00:00

abstract：:The concentration of high-affinity estradiol binding sites in the uteri and pituitaries of normal and two dwarf strains of mice was studied. The two dwarf strains are characterized by their total absence of growth hormone or prolactin synthesis and low or absent TSH production. The uteri of the dwarf mice are small bu...

journal_title：Molecular and cellular endocrinology

authors： Sartor P,Slabaugh M,Sakai D,Gorski J

更新日期：1983-01-01 00:00:00

abstract：:The present study was aimed at characterizing the deficiency of the glucocorticoid-receptor system in the immature pituitary gland of neonatal rats. Under conditions of both constant protein and DNA concentrations, [3H]dexamethasone binding to nuclei was significantly lower in the immature, compared with the mature, g...

journal_title：Molecular and cellular endocrinology

authors： Sakly M,Koch B

更新日期：1982-09-01 00:00:00

abstract：:Several forms of phosphodiesterase are present in the male gonad, and their relative concentrations vary during testicular development. On the basis of kinetic analysis and chromatography on DEAE cellulose, 3 forms were separated: (a) a high-affinity cGMP phosphodiesterase, regulated by Ca2+ and calmodulin, similar to...

journal_title：Molecular and cellular endocrinology

authors： Geremia R,Rossi P,Pezzotti R,Conti M

更新日期：1982-09-01 00:00:00

abstract：:Granulosa cells were isolated from antral follicles of intact, adult female rats by treatment with EGTA and hypertonic sucrose, and were kept in culture for 1, 15 or 37 days. The amount of inhibin-like activity in spent rat granulosa-cell media (rGCCM) was assessed by using a bioassay for inhibin (measurement of the u...

journal_title：Molecular and cellular endocrinology

authors： Hermans WP,van Leeuwen EC,Debets MH,Sander HJ,de Jong FH

更新日期：1982-08-01 00:00:00

abstract：:Cultured rat anterior pituitary cells were continuously perfused with medium 199, and exposed to short (5 min), intermediate (30 min), or long (6 h) pulses of a maximally effective concentration of gonadotropin-releasing hormone (GnRH). Assay of the effluent by radioimmunoassay and interstitial-cell bioassay revealed ...

journal_title：Molecular and cellular endocrinology

authors： Naor Z,Katikineni M,Loumaye E,Vela AG,Dufau ML,Catt KJ

更新日期：1982-07-01 00:00:00